Bone Cancer Research Today is a free monthly online journal that collates and summarizes the latest research about Bone Cancer, including details on symptoms, types, treatment.

Gene expression profile in oral squamous cell carcinoma: a pilot study.

Somoza-Martín JM, García-García A, Barros-Angueira F, Otero-Rey E, Torres-Español M, Gándara-Vila P, Reboiras-López MD, Blanco-Carrión A, Gándara-Rey JM

Universidad de Santiago de Compostela, Complejo Hospitalario, Universitario de Santiago, Spain.

PURPOSE: To study the gene expression profile of oral squamous cell carcinomas. MATERIALS AND METHODS: Gene expression profile was investigated in oral squamous cell carcinomas in 5 patients using the Atlas Glass Human 3.8 I Microarray (which detects cDNA obtained from cellular total RNA) (Clontech Laboratories, Palo Alto, CA). Data were normalized by the LOWESS method. Statistical significances of deviations from a 1:1 ratio were evaluated by t tests, with P<.05. RESULTS: Of the 3,757 genes analyzed, 322 (8.6%) were significantly overexpressed in tumoral tissue with respect to normal tissue, while 104 (2.8%) were significantly underexpressed. The affected genes fell into a wide range of functional categories. CONCLUSION: We consider that cDNA microarrays are of clear value for investigating the biology of these tumors, and that this technology may help in the molecular classification of oral squamous cell carcinomas and in the identification of targets for gene therapy.

Published 9 June 2005 in J Oral Maxillofac Surg, 63(6): 786-92.
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